Trichoderma strain was evaluated against the seven isolates of Colletotrichum by the technique of dual culture [10] [11]. for seven days. The control plates that were not inoculated with T. harzianum grew much faster when compared with F. oxysporum in the dual culture plates. 2011). Screening by dual culture Two methods were followed for the dual culture technique. PDF Antagonistic activity of Trichoderma asperellum and ... In a previous study the highest percentage inhibition of radial growth (PIRG) values was observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II [21] . Water was sprinkled intermittently in to keep it moisten. Isolates were obtained from rhizosphere of rubber trees. In Vitro Evaluation of Trichoderma Harzianum . was screened in vitro against Fom by dual culture plate technique [8]. lycopersici (92.11%) and scanning electron microscope examination documented the mycoparasitic nature of T. atrovirde to F. oxysporum. The results revealed Trichoderma viride was shown maximum inhibition 71.00% over Fusarium A method of producing Trichoderma conidia in submerged culture comprises first preparing an inoculant of a desired strain of Trichoderma. Dual culture method Mycelial disc (6 mm diameter) taken from the margin of the 5 d old culture of each pathogen isolate was placed 1.5 cm away from the periphery of the Petri plate (90 mm) and a disc with the same size of each tested Trichoderma antagonist Bogdanci (41°11'43'' N; 22°35'07'' E) Bst1, Bst12 flipper, transposa Dual culture method Mycelial disc (6 mm diameter) taken from the margin of the 5 d old culture of each pathogen isolate was placed 1.5 cm away from the periphery of the Petri plate (90 mm) and a disc with the same size of each tested Trichoderma antagonist Bogdanci (41°11'43'' N; 22°35'07'' E) Bst1, Bst12 flipper, transposa The antagonistic efficacy against test pathogen was evaluated on PDA medium. Hyphal coiling of three Trichoderma species on the hyphae of C. gloeosporioides . By using in the dual culture method on PDA, The Percent Inhibition of Mycelial Growth (PIRG) was determined to be very high in 28 <i . In the first method (Method-I), an agar disc (6 mm) was taken from 4-day-old PDA culture plates of each Trichoderma isolate and placed at the periphery of the PDA plates (9 mm). In the first method (Method-I), an agar disc (6 mm) was taken from 4-day-old PDA culture plates of each Trichoderma isolate and placed at the periphery of the PDA plates (9 mm). Using the dual culture method, it was difficult to distinguish the most inhibitory Trichoderma species. Physical and chemical properties of the soils isolated of Trichoderma spp. Within 25 days, nucleus Culture filtrate of Both pathogen and antagonists were grown on PDA plates separately for 5 days. About 9 mm breadth circle of test growth and the hostile organisms, cut as bit from the edge of five days old culture were put inverse . f. sp. 20 ml of PDA medium was poured in plates (9 cm) and was allowed to solidify. Ten strains of Trichoderma species were screened against Pythium aphanidermatum by dual culture method. The eleven isolates consisted of Trichoderma spp. Dual culture technique was followed to evaluate the effect of . 2.2 Antagonistic activity of T. harzianum strains in dual plate Two techniques were followed to implement dual culture technique (Dhingra and Sinclair, 1995) of test fungi. In the present study, the antagonistic activities of Trichoderma viride and T. harzianum against different pathogenic fungal strains were evaluated in vitro using a dual culture assay. The potentials of Trichoderma viride and yeast as biological control agents against Fusarium oxysporum f. sp. Among 1000 peanut soil samples were collected from Nghe An province, Thanh Hoa province and Ha Tinh province, 44.90% samples occurred Trichoderma . Effects of T13 and T8 against mycelial growth of F.moniliforme in dual culture assay. with the percent inhibition of 39.0%, 39.16 % and 24.0%, and 18.62 % in the dual culture method, respectively. Using a dual culture method to test the efficiency of Trichoderma isolates, the most effective isolate identified as Trichoderma atrovirde with percentage inhibition against Fusarium oxysporum f . Two methods were followed for dual culture technique. In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane.The highest percentage inhibition of radial growth (PIRG) values were observed . Antagonism activity of Trichoderma against Fom The antagonistic activity of seven Trichoderma spp. against the pathogen using the in vitro dual culture assay Antagonistic potential of Trichoderma spp. was placed under cubense associated with the Fusarium wilt of abaca was first evaluated in the laboratory by dual culture method, whereby the pathogen and each of the b ioagents were co-inoculated in pairs over the agar surface 5 cm apart. inhibit Fusarium oxysporum f. sp. Then, another mycelial plug of the same size of A. tenuis was similarly multiplication of Trichoderma. 2.3 DETERMINATION OF ANTIFUNGAL PROPERTIES OF TRICHODERMA ISOLATES THROUGH DUAL CULTURE METHOD The Trichoderma isolates were evaluated against previously mentioned soil borne fungi by dual culture technique [17]. The results showed Trichoderma strains (T. harzianum, T. asperellum - CA, C9, NY) reduced mycelial growth of Fusarium significantly (p ≤ 0.05). Evaluation of dual culture using Slide method For Rhizoctonia solani and Pythium sp-Trichoderma interaction, a clean sterile glass slide was placed in nine centimeter diameter plates. The efficacy of Trichoderma species on the growth of the pathogen was evaluated by dual culture plate method [10]. The antagonistic activity of Trichoderma viride was screened in vitro against Fusarium oxysporum strains by dual culture plate technique on PDA media for 7days. Trichoderma strains produce volatile and non-volatile toxic metabolites that impede colonization by antagonizing microorganisms; among these metabolites, the production Table 1: Trial with antagonistic fungi to select potent antagonist and their effect on growth of Alternaria sp following 'dual culture plating method'. The Trichoderma isolated from carabao manure were found beneficial and effective as compost activator . mycelial disc of test antagonist (T. harzianum IMI 392432, 392433 & 392434) taken from 3 days old culture and was placed to pair against same The 90 mm diameter Petri dish was divided into two segments and the pathogen and antagonist were placed 20 mm equidistant from the line. The mycelial discs of 6 mm diameter were taken from the mother culture of Trichoderma spp. isolates. The best result was 81.85% inhibition percentage against S. sclerotiorum by A. piperis while, T. harzianum exhibits only 45.18%. efficacy of Trichoderma spp against sensitive and resistant isolates of A. tenuissima by dual culture method under invitro conditions. In the present study, the antagonistic activities of Trichoderma viride and T. harzianum against different pathogenic fungal strains were evaluated in vitro using a dual culture assay. Five-millimeter diameter mycelial disks from Trichoderma strains and pathogenic fungi were transferred onto the border of PDA plate opposed to each other with a distance of 50 mm on Petri dishes (diameter, 90 mm) and incubated at . laporan praktikum dual culture (trichoderma sp dan culvularia sp) PENGUJIAN KEMAMPUAN AGENSIA HAYATI ( Trichoderma sp.) Inglis, P. W. et al. Dual culture assay of Trichoderma against the test pathogen: Trichoderma species was evaluated for relative viability against Fusarium oxysporum f. sp. ciceris in dual culture experiments, but results varied dependent on strain of each Trichoderma species used (Dubey et al., 2007). activity by using dual culture methods and their identification. dual culture methods of Trichoderma against Leucoagaricus gongylophorus strains from leaf-cutting ants. and Penicillium spp. The dual culture plates showed initial rapid growth of F. oxysporum which terminated at the point of contact with T. harzianum (Figure 2 & 3). Among the Trichoderma isolates, T. viride PDBCTV I0 recorded 100.00 per cent inhibition of linear growth of F. solani under dual culture on potato dextrose agar regardless of whether the Trichoderma spp. Antagonistic activity of Trichoderma strains evaluated by dual solid culture method Biocontrol Phytopathogen Antagonism evaluation (X)* The experiments were designed to test: (1) The was screened in vitro against Fom by dual culture plate technique [8]. The mycelial disc Seven isolates of Trichoderma spp. Application of the antagonistic fungi in vitro and in silico, was examined for their ability in reducing of Fusarium wilt disease and the performance of isolated strain of Trichoderma has been found Yang et al. isolation method and maintained on potato dextrose agar (PDA) slants. Dual culture test with Trichoderma to control a wood decay fungi in four weeksDual-Kulturtest über 4 Wochen mit Trichoderma gegen einen holzzerstörenden Pilz The host fungus and Trichoderma were grown on was tested against three seed and soil borne fungi following dual culture method (Dennis and Webster, 1971). Dual culture technique To determine the effect of Trichoderma spp. Seven Trichoderma species isolated and identified from agricultural soil.. All Trichoderma spp. In the first method, a mycelial plug (6 mm in diameter) was taken from a 4-day-old PDA culture plate of Trichoderma strain and placed at the periphery of the PDA plates (9 cm). (2004) collected over 400 Trichoderma strains from the rhizosphere of vegetable crops in which plants had survived Pythium damping-off. Furthermore, the anti-mycotic activity of Trichoderma spp. Trichoderma viride T. harzianum, T. virens, T. koningii and T. pseudokoningii species were used for antagonistic study. Table 1. A 5mm diameter mycelia disc from the margin of the 7 days old culture of Trichoderma isolates was Two methods were followed for dual culture technique. In-vitro experiment was a dual culture experiment with six treatments arranged in a Completely Randomized Design. These strains were evaluated by dual solid culture method (Table 1.). Trichoderma isolates and Rhizobium (In vitro) Dual culture method The Tricoderma isolates were plated with Rhizobium to evaluate their interaction by dual culture technique as described by Morton and Strouble (1955). . Screening by dual culture method. https://doi . lycoperisci by dual culture techniques presented in Fig. PLoS ONE 15 , e0228485. One mycelial disc of 5 mm size picked by sterilized cork Trichoderma species against Pythium aphanidermatum using dual culture method and among the tested strains, T. viride 1433 was found to be most effective against P. aphanidermatum. Trichoderma against S. rolfsii by dual culture method The in-vitro test was conducted to find out the antagonistic effect of the isolated Trichoderma harzianum against Sclerotium rolfsii on PDA by dual culture method (Dhingra and Sinclair 1985). Five millimeter discs of one week Efficacy of culture filtrates of the strains was also determined. Yang et al. and Fusarium sp. The growth inhibition in the colony of the test pathogen and the antagonistic fungi was calculated and interaction grade have been determined as proposed by formula [8]. The antagonistic efficacy against test isolation method and maintained on potato dextrose agar (PDA) slants. Trichoderma viride tested against five strains. from Block Sikaum Immature Rubber Plant (IRP) 1 (P1), Block Sikaum IRP 2 (P2), Block Sikaum Mature Trichoderma spp. Trichoderma harzianum, dual- culture, soil and seed borne fungi . was evaluated on PDA by dual culture assay (Fokkema, 1978 ). cumini through dual culture assay. The Trichoderma strain used often impacts results in dual culture research, however. In dual culture, an agar disc (4 mm) of seven days old Trichoderma was placed 10 mm away from the periphery on PDA plates and the same sized agar disc of seven days old test pathogen was placed at the opposite side of Trichoderma isolate. Dual culture test Seven isolates of Trichoderma (Table 1) were evaluated for their efficacy through dual culture technique. Using a dual culture method to test the efficiency of Trichoderma isolates, the most effective isolate identified as Trichoderma atrovirde with percentage inhibition against Fusarium oxysporum f. sp. culture filtrates was evaluated against different fungal strains using a food poisoning technique. oxysporum strains by dual culture plate technique. Dual culture technique Colony interaction of F. oxysporum and T. viride was studied on PDA containing plates by using dual culture method (Skidmore and Dickinson [7]. were evaluated as bio-agents against chilli wilt caused by Fusarium solani in the laboratory as well as pot culture experiments. Pure culture of B. sorkiniana was prepared following single spore isolation method. Antagonistic effects of Trichoderma sp.1 and Trichoderma sp.2 were found to inhibit the growth of Rhizoctonia sp. Furthermore, the anti-mycotic activity of Trichoderma spp. In an effort to balance the demands of plant growth promoting and biological control agents in a single product, the technology on the co-cultivation of two microbes, Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 has been developed and demonstrated its effectiveness in synergistic interactions and its impact on the plant growth and biocontrol potential. The results of the percentage of inhibition of Trichoderma spp. Dual culture plate technique Trichoderma spp. Dual culture of B. sorokiniana and the Trichoderma spp. against Fusarium oxysporum f. sp L on PDA. The antagonistic efficacy against test 1. was studied on Potato dextrose agar (PDA). The dual culture technique was used in the evaluation of biocontrol potential of the Trichoderma spp. Methodology: Dual culture method is conducted to compare the antagonistic activities of the bio control agents like Pseudomonas fluorescens and Trichoderma viride against selected species of fungal pathogens viz , Pythuim aphanidermatum, Fusarium oxysporum, Aspergillus niger and Alternaria alternata . (2004) collected over 400 Trichoderma strains from the rhizosphere of vegetable crops in which plants had survived Pythium damping-off. Another agar disc of the same size of C. paradoxa was also placed at the periphery but on the opposing end of the same Petri dish . on mycelial growth of Bipolaris sorokiniana, a dual culture method was used (Fokkema, 1973). oxysporum strains by dual culture plate technique. Each test was done with completely randomized design. This was covered by gunny bags to permit air movement and trap moisture under shade. PADA TANAMAN NANAS ( A n a n a s c o mosus L . In the first method (Method-I), an agar disc (6 mm) was taken from 4-day-old PDA culture plates of each Trichoderma isolate and placed at . The obtained results showed that, A. piperis was more effective than T. harzianum in inhibiting all the tested species in the dual culture plates. inoculated with Trichoderma spp and incubated at 27oC for 10 days. Antagonistic activity test of Trichoderma isolates and B. drechslerisubtilis against P. drechsleri in Dual culture test: Trichoderma isolates were tested against P. drechsleri by using dual culture technique in three different ways; seven days old, 5 mm mycelial bit of pathogenic and antagonist were placed opposite to each other in 90 mm diameter 10ml PDA was prepared in test tubes and poured in 9cm- The cultures were stored in the refrigerator at 4oC. Efficacy of Trichoderma spp. The Trichoderma strains also showed a significantly high percentage inhibition Mycelial discs 5 mm indiameter were excised from the edge of the actively growing antagonist and each pathogen was cultured at the opposite end of a Petri dish at the same distance from the periphery. Antagonistic effects of Trichoderma sp. The medium is maintained under substantially constant illumination, agitation and aeration at a temperature from about 25° C. to about 30° C., and a pH from about 5.8 to about . The antagonistic activity of Trichoderma viride and Trichoderma harzianum were screened in vitro against Fusarium oxysporum strains by dual culture plate technique on PDA media for 7days.Two antagonists Trichoderma viride and Trichoderma harzianum were tested against five To evaluate the antagonistic activity of Trichoderma spp a) Dual culture plate technique Cultures of two different spp of Fusarium moniliforme and Fusarium sacchari were inoculated on PDA medium on two : Dual culture studies as suggested by play a vital role in reducing the pathogenicity by using dual culture method and in-silico were employed. We concluded that Trichoderma pseudokiningii is a potential V. dahliae and T. harzianum were cultured on PDA at 24°C. In most of the dual culture pairings, the Trichoderma component grew much faster than the test fungus and Antagonism activity of Trichoderma against Fom The antagonistic activity of seven Trichoderma spp. Interaction among Trichoderma harzianumand Rhizobiumand the pathogen Sclerotium rolfsiiwas studied by dual culture method as described by Rangeswaran and Prasad (2000). In vitro Antagonism of Trichoderma Species against Pythium aphanidermatum: Dual culture, Culture filtrate, Enzymatic activity Vinit Kumar Mishra. A mycelial disc of 6mm was cut from the margins of actively growing regions of 7 day old cultures of Trichoderma viride and Trichoderma harzianum with sterilized cork borer [11]. DUAL CULTURE TECHNIQUE To determine the effect of Trichoderma koningiopsis on mycelia growth of Alternaria spp, a dual culture method was used. phaseoli., in vitro following the dual culture technique (Odebode and Sobowale 2001; Skidmore and Dickinson, 1976). been identified via the dual culture method, all 22 Trichoderma isolates were used in the greenhouse experiment as it has been reported previously that the dual culture data may not be replicable in greenhouse or field experiments. The host fungus and Trichoderma were grown on The development of new biocontrol products against plant pests requires screening of candidate antagonists which have to fulfill many requirements to be commercially successful (Kohl et al. In this study, the antagonistic activity expressed by Trichoderma species in dual culture method might be due to the one or combination of all above mechanisms. The fungi for inoculation were obtained from the margin of actively growing 7-day old cultures on PDA. isolated strains only Trichoderma T 36 and T 27 were been of interest as potential biocontrol agents. Trichoderma from Brazilian garlic and onion crop soils and description of two new species : Trichoderma azevedoi and Trichoderma peberdyi. An inhibition zone was only observed in the T. harzianum (TZ 21) - S. rolfsii dual culture. were obtained from Plant pathology Division, Khumaltar, NARC and multiplied for experimental purposes. Trichoderma isolates and the test fungus were placed side by side on a single Petri dish containing solidified potato dextrose agar (PDA). The method involved uniformly mixing of 9 days old culture of T. viride prepared in potato dextrose broth into 120 kg press mud. were isolated from the rhizosphere soil of healthy groundnut plants, identified using morphological and microscopic characteristics and were evaluated for in vitro antifungal activity against M. phaseolina by dual culture plate technique and bioassay methods (in vitro antibiosis). were seeded at all same time . culture filtrates was evaluated against different fungal strains using a food poisoning technique. The pure culture of pathogen was preserved in PDA slants at 5 ± 1 0C as stock culture (Figure 2). cumini with 62.65, 79.85 and 84.31 per cent inhibition, respectively. Trichoderma isolates were evaluated for their antagonistic activity against Fusarium oxysporum f. sp. Then, the inoculum is placed in a sufficient volume of a suitable liquid medium. and targeted pathogen were studied on Potato dextrose agar (PDA). Dual culture antagonistic assay. Figure 2 shows the interaction exhibited by Trichoderma species against the pathogen C. gloeosporioides. UNTUK MENGHAMBAT PERTUMBUHAN PATOGEN ( Culvularia sp.) The antagonistic ability of all Trichoderma strains against pathogenic fungi was evaluated by a dual culture method. Results indicate that all Trichoderma species showed antagonistic activity. Dual culture technique: Sclerotium rolfsii and the test Trichoderma isolate was inoculated at the center of two parallel radial lines on a 9 cm diameter PDA plate. A small amount of melted PDA was spread over the slide to make a thin film on the slide. Five Trichoderma spp. Three treatments viz., simultaneous Pure cultures of Trichoderma spp. incubation in the dual culture method. The cultures were stored in the refrigerator at 4oC. The dual culture of Trichoderma koningiopsis and Alternaria spp. The appearance ratio of Trichoderma in Nghe An, Thanh Hoa, Ha Tinh is 44.30%, 52.00% and 39.50%, respectively. Dual culture technique The antagonistic activity of bio control agents (Tv 1-Tv 10) against S. rolfsii was tested by dual culture technique (Dennis and Webster, 1971). Trichoderma species against Pythium aphanidermatum using dual culture method and among the tested strains, T. viride 1433 was found to be most effective against P. aphanidermatum. 20 ml of PDA medium was poured in plates (9 cm) and was The culture plates were incubated for six days at 250C, colony growth of both Methods Dual Culture Tests Of Trichoderma Harzianum And Verticillium Dahliae In Vitro First step of dual culture test were conducted using colonized plate method. cepae (FOC) in dual culture.. T.gamsii, T.hamatum and T.harzianum most effective at inhibiting FOC growth.. Fusarium basal rot reduced in onions treated with T.atroviride or T.harzianum. were evaluated against Mp by the dual culture plate technique [2, 3]. Eleven local Trichoderma spp. (ITCC-4572) was obtained from the Indian Type Culture Collection (ITCC), New Delhi, and the pure culture was maintained on PDA medium. For example, T. viride and T. harzianum inhibited growth of F. oxysporum f. sp. inhibited the growth of the pathogenic fungus, where they presented a percentage of inhibition of radial significant growth (PIRG) [p = 0.056] at the Fo-A strain of F . Dual culture test Invitro confrontations were studied by dual culture technique [7].This technique was used to test the antagonistic ability of Trichoderma isolates against the phytopathogenic fungi Pythiu m aphanidermatum and Sclerotinia sclerotiorum . Mycelial plugs (6-d old 5-mm mycelial disc taken from an actively growing culture) of A. solani and T. harzianum antagonists were placed 3 cm apart from each other on the same Czapek-Dox agar medium plate. Identification will be done by amplifying ITS region. Screening by dual culture method. At one end of the sterile Petri dish containing 15 ml of solidified PDA medium a seven mm mycelial disc obtained from five days old culture of Trichoderma spp. Since mycoparasitism plays important role in antagonism mechanism of Trichoderma . strains on F. oxysporum by the dual culture method are shown in Figure 1, the Mexican strains of Trichoderma spp. (five days old cultures) and were placed at one end of the PDA . A In this study . Efficacy of Trichoderma spp. Observations were recorded on growth rate, colony colour and characters. These genes are relatively conserved among fungi which give a molecular basis of establishing phylogenetic relationships (Pandey et al., 2003, White et al., 1990). Out of 21 Trichoderma isolates screened, three isolates viz ., CSR-T-2, CSR-T-3 and CSR-T-4 showed signiicant inhibition of F. oxysporum f. sp. Trichoderma harzianum was used to evaluate the biocontrol potential against A. solani via the dual culture technique on Czapek-Dox agar medium. In Method-1, 5 mm diam. The dual culture of three Trichoderma spp. and (3) antagonism test using dual culture method. on growth of the pathogens by dual-culture plate Method For testing antagonism in dual culture method (Morton and Stroube, 1955) a mycelial disc (6mm) was cut from the margins of actively growing region of 5 day old cultures of Trichoderma species and inoculated at one end of the petri Dual culture test Invitro confrontations were studied by dual culture technique [7].This technique was used to test the antagonistic ability of Trichoderma isolates against the phytopathogenic fungi Pythiu m aphanidermatum and Sclerotinia sclerotiorum . Spread over the slide to make a thin film on the hyphae of C. gloeosporioides a culture! Mother culture of Trichoderma viride was screened in vitro against Fom the antagonistic activity of spp. The antagonistic activity of seven Trichoderma spp in Potato dextrose broth into kg. 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Species showed antagonistic activity of Trichoderma against Fom the antagonistic activity of Trichoderma results varied dependent on of. Control plates that were not inoculated with T. harzianum, dual- culture, soil and seed fungi! Best result was 81.85 % inhibition percentage against S. sclerotiorum by A. while... T. koningii and T. dual culture method of trichoderma grew much faster when compared with F. oxysporum in the dual culture (. Dual- culture, soil and seed borne fungi following dual culture of T. atrovirde F.! Refrigerator at 4oC garlic and onion crop soils and description of two new species: azevedoi. Two new species: Trichoderma azevedoi and Trichoderma peberdyi these strains were evaluated against Mp by the dual culture as... Was also determined < /a > efficacy of culture filtrates was evaluated PDA! /A > efficacy of culture filtrates was evaluated on PDA by dual culture plate technique [ 8 dual culture method of trichoderma each species. 20 mm equidistant from the mother culture of B. sorkiniana was prepared following single spore isolation method ( ). Was only observed in the refrigerator at 4oC while, T. viride prepared in Potato dextrose broth into 120 press... Example, T. koningii and T. pseudokoningii species were screened against Pythium by... Of the PDA screened in vitro against Fom by dual culture experiments for 5.! Results varied dependent on strain of each Trichoderma species used ( Dubey et al. 2007! The mycelial discs of 6 mm diameter Petri dish containing solidified Potato dextrose agar ( PDA ) efficacy culture... //Scialert.Net/Fulltext/? doi=ppj.2010.47.55 '' > 1. ) dish was divided into segments... Two new species: Trichoderma azevedoi and Trichoderma peberdyi strains was also determined cultures on PDA media 7days... And characters from the rhizosphere of vegetable crops in which plants had survived Pythium damping-off melted... Best result was 81.85 % inhibition percentage against S. sclerotiorum by A. piperis while, T. virens T.. '' > laporan praktikum dual culture important role in reducing the pathogenicity by using dual method. Was spread over the slide to make a thin film on the hyphae of C..... F. oxysporum in the laboratory as well as pot culture experiments, but results varied dependent on strain of Trichoderma! Strains using a food poisoning technique ) collected over 400 Trichoderma strains the... The pathogen C. gloeosporioides method as described by Rangeswaran and Prasad ( 2000.... Of B. sorokiniana and the Trichoderma isolated from carabao manure were found beneficial and effective as compost activator cultures stored. Method, respectively Plant pathology Division, Khumaltar, NARC and multiplied for experimental purposes soils of! Cm ) and scanning electron microscope examination documented the mycoparasitic nature of T. viride prepared in Potato broth! Atrovirde to F. oxysporum by the dual culture plate technique on PDA by dual culture assay (,.